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MesoScale Discovery (MSD) assays at Crux Biolabs

MesoScale Discovery (MSD) immunoassays are used to measure the concentration of proteins or other molecules in biological samples.

They detect multiple analytes in a single sample and are superior to Luminex because of a wider dynamic range, reduce matrix effects and increase sensitivity. MSD assays have become an important tool in clinical trials research, as they allow our scientists to quantify a variety of molecules, from cytokines and growth factors to hormones and antibodies, in complex biological matrices like blood, serum, and plasma.  

So, how does an MSD assay work? The process begins by coating a plate with capture antibodies specific to the analytes of interest. The plate is then incubated with the biological sample, allowing the target molecules to bind to the capture antibodies. Next, detection antibodies labelled with electrochemiluminescent (ECL) tags, which bind to the captured analytes. When an electric current is applied to the plate, the ECL tags emit light, which is detected by a sensitive photomultiplier tube. The amount of light emitted is directly proportional to the amount of analyte present in the sample, allowing for quantification of the target molecules and thus, our results.  

One of the major advantages of MSD assays is their ability to detect up to 10 analytes in a single sample. This allows our researchers to investigate the relationships between different molecules and to examine complex biological pathways that involve multiple signalling molecules. MSD assays are also highly sensitive, with a wide dynamic range that can detect molecules at very low concentrations. Additionally, MSD assays require only small sample volumes, making them an attractive option for those precious samples. 

Another advantage of MSD assays is their versatility. There are a variety of different MSD assays available, each designed to detect different sets of analytes. This allows researchers to select the assay that is most appropriate for a particular research question. In addition, MSD assays are highly customisable, allowing us to design assays tailored to their specific needs.  

Overall, MSD assays have become an indispensable tool in our – and all – biological research and we predict as said research “evolves” (excuse the pun), MSD assays will undoubtedly play an important role in advancing our understanding of complex biological systems. To read more about MesoScale Discovery at Crux, see our MSD capabilities here.

If you’d like to discuss more, get in touch with our team about how MesoScale Discovery may help you through this link.