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The importance of PBMCs in bioanalysis

Peripheral blood is a key component in many clinical studies. From a single blood draw, multiple blood components can be isolated for numerous downstream applications.

Changes in treatment responses can be measured with assays designed to detect biomarkers in circulating tumour DNA, serum or plasma, while cellular responses of peripheral blood mononuclear cells (PBMCs) can be analysed in various cell-based assays, such as restimulation to measure cell-mediated immunity or potency assessment of new drugs.

At Crux Biolabs, we support sponsors in making informed decisions on isolation of PBMCs and whole blood components for their studies.

Our scientific team discuss the specificities of each isolation method, such as processing time, yield and purity. We then design a preparation method that is bespoke to a sponsor’s needs. We are experts in different methods of PBMC processing and separation techniques, including traditional density gradient (overlay and underlay), customised isolation tubes (SepMate and LeucoSep), and Cell Preparation Tubes (CPT). Isolated PBMCs can then be cryopreserved in liquid nitrogen, lysed for specific protein application, or further fractionated to obtain purified populations for downstream cell applications, such as the production of CAR-T cells.

We can provide cell counts and viability of study samples before and after cryopreservation, as a sign of confidence in the quality of our sample preparation process. Fitness of the PBMCs can also be confirmed by performing cell-based assays such as ELISpot.  We do this to offer the maximum transparency to sponsors.

Crux Biolabs is proud to offer a global PBMC processing service through our sites in Melbourne and Sydney. We are well-positioned to support clients in resolving challenges surrounding clinical studies.

Crux Biolabs also offers flexible storage arrangements, with cryopreservation available when required. All cryopreserved samples are logged using an electronic sample management system to offer maximal traceability. These cryopreserved samples can be further processed in various downstream applications and services at Crux Biolabs, such as Flow Cytometry, ELISpot, neutralisation assays and transduction assays. Alternatively we can organise shipment of samples to third parties. Our bioanalytical expertise can help to qualify stability windows and quality requirements.

We are proud to provide comprehensive training to our scientific team. This has been recognised by independent external bodies such as NATA and IVRN, as well as successful qualification processes conducted according to sponsors requirements. In addition, Crux Biolabs also has expertise in training external sites internationally, ensuring optimal harmonisation of multisite studies.