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Flow Cytometry

Flow
Cytometry

Flow Cytometry enables the detection and quantification of markers on Immune Cells and FACS Analysis. Our Crux Biolab’s Flow Cytometry services are performed on CytoFLEX Platform instruments with up to 21 colors. Flow cytometry is widely used in research and clinical settings for applications such as cell counting, cell sorting, and biomarker detection. It can be used to analyze a wide range of cell types, including bacteria, yeast, and mammalian cells.

Crux provides a range of flow cytometry assays including:

  • Immunophenotyping including intracellular markers
  • Intracellular cytokine staining
  • CAR-T cell characterization for cell therapy development
  • Receptor occupancy (RO) measurement
  • Internalisation assays
  • Antibody-dependent cellular phagocytosis assays
  • Phagocytosis assays
  • Phospho-flow cytometry
  • Tetramer assays
  • More about flow cytometry
    Details about the process of flow cytometry

    Flow cytometry is a technique used to analyse and sort cells or particles in a liquid suspension. It utilises a flow cell, where the cells or particles are passed through one or multiple laser beams, which excites the fluorescent dyes that are attached to specific cell structures or markers. The resulting light emissions are then analysed by a detector, which can provide information on the size, shape, and fluorescence intensity of the cells or particles. This information can be used to identify and isolate specific cell types or populations based on their unique characteristics.

     

    The technique is widely used in research and clinical settings with Crux, for a variety of applications, including cell counting, cell sorting, protein and gene expression analysis, and detection of cellular abnormalities. Flow cytometry is also used in diagnostics for the detection of cancer, infections and other diseases. Many people credit it as revolutionising the way scientists study cells and has become an essential tool in many areas of biomedical research, enabling researchers to better understand the underlying mechanisms of diseases and contributing to the development of new therapies.

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